【摘要】 目的研究甲基对硫磷对雄性大鼠的睾丸毒性作用,探讨其毒理作用机制。方法选择雄性SD大鼠(220-240g)20只,连续6周灌胃染毒,剂量为1.2,6,30mg/(kg.bw)染毒结束次日处死大鼠。用流式细胞技术分析睾丸细胞周期和细胞凋亡,同时检测睾丸组织超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量。结果1.2,6,30mg/(kg.bw)甲基对硫磷染毒后大鼠精子存活率分别为90.40%,82.99%,73.98%;精子畸形率分别为20.88%,23.91%,29.10%,与对照组相比较差异有统计学意义(P<0.05或(P<0.01)。30mg/kg染毒组的SOD活力185.33U(mg.pro),低于阴性对照组[344.61U/(mg.pro)];MDA含量1.90nmol/(mg.pro)高于其阴性对照组(0.71nmol/mg.pro)(P<0.01)。与对照组比较,中、高剂量染毒组G0/G1期细胞比例(73.43%,75.22%)显著降低,S期细胞(12.10%,9.35%)比例降低;高剂量甲基对硫磷组细胞凋亡率(32.77%)高于对照组(22.17%),差异有统计学意义(P<0.05)。结论甲基对硫磷对大鼠具有明显的生殖毒性,其毒理作用机制可能是氧化应激导致细胞周期的改变和细胞凋亡的增加。更多还原

【Abstract】 Objective To investigate the effect and possible mechanisms of methyl parathion on testis cell cycle and cell apoptosis in male rats.Methods Twenty SD male rats(220～240 g) were divided into four groups.The methyl parathion(MPT) was given orally to the rats for six weeks at the dose of 1.2,6,30 mg/kg/day,respectively.The testicular viscera coefficient was measured and the activity of superoxide(SOD),the contents of maleic dialdehyde(MDA) were detected.The flow cytometry was used to study the effects of MPT on testicle cells of male rats.Results After the exposure of 1.2,6,30 mg/kg MPT,the sperm survival rates were 90.40%,82.99%,73.98% and the sperm deformity rates were 20.88%,23.91%,29.10%.Compared with the control group there were statistically significant changes(P<0.05,P<0.01).In 30 mg/kg group,the activity of SOD(185.33 U/mg·pro) was lower that that of negative control group(344.61 U/mg·pro)and MDA content(1.90 nmol/mg·pro) was higher than that of negative control group(0.71 nmol/mg·pro,P<0.01).Compared with the control group,the proportion of cells in G0/G1 phase(73.43%,75.22%) and S-phase cells(12.10%,9.35%) were significantly lower in the middle and high dose group.In the high-dose group the apoptosis rate(32.77%) was higher than that of control group(22.17%,P<0.05).Conclusion The toxic effect of MPT on testicles of the rats is obvious,the oxidative stress may lead to changes in cell cycle and increase of apoptosis.更多还原