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大连市伤寒沙门菌扩增片段长度多态性分型
Genotyping of Salmonella typhi sampled in Dalian area by amplified fragment length polymorphism
【摘要】 目的运用扩增片段长度多态性(AFLP)技术分析辽宁省大连市2000~2006年伤寒沙门菌分离株的多态性及遗传关系。方法采用PstI/Mse I型内切酶,选择9种引物组合对30株不同来源的菌株进行AFLP分子分型。结果30株不同来源的伤寒沙门菌分为29个AFLP基因型,呈现丰富的多态性。结论AFLP技术是一种兼具有效性和效率性,适用于伤寒沙门菌分子流行病学研究。
【Abstract】 Objective To analyze the intraspecific genetic relationship and polymorphism of the Salmonella typhi strains isolated from stool samples collected in Dalian area during 2000～2006.MethodsAmplified fragment length polymorphism(AFLP) was applied to 30 Salmonella typhi isolates using the restriction enzymes Pst I/Mse I and 9 selective primer pairs.ResultsTwenty-nine different AFLP gene types were found among the 30 strains of Salmonella typhi.ConclusionAFLP is a valid and efficient method to research the molecular epidemiology of Salmonella typhi.
【Key words】 amplified fragment length polymorphism(AFLP)； Salmonella typhi； molecular typing；
- 【文献出处】 中国公共卫生 ,Chinese Journal of Public Health , 编辑部邮箱 ,2009年01期
- 【分类号】R378.22
- 【下载频次】106