【摘要】 目的:观察活血化瘀中药对激素性股骨头缺血性坏死大鼠转化生长因子β1(TGFβ1)表达的影响,进一步探讨其防治激素性股骨头坏死的作用机制。方法:采用清洁级SD大鼠40只,随机分为空白组4只和模型组36只,模型组每周2次腹腔注射醋酸泼尼松龙24.5mg/kg,经6周诱导出早期激素性股骨头缺血坏死的模型,空白组每周2次腹腔注射同等剂量的生理盐水。6周后处死空白组4只和模型组4只,进行光、电镜观察,确定造模成功。随后将其余32只模型组大鼠再随机分为治疗组16只和对照组16只,分别灌服桃红四物汤和生理盐水,于灌胃后第6、8周检测血清中TGFβ1的含量;检测股骨头局部TGFβ1mRNA的转录及股骨头局部TGFβ1的表达。结果:①图像分析仪分析:治疗组股骨头局部TGFβ1较对照组表达明显增强,两组有明显差异(P<0.01)。②血清TGFβ1含量检测:治疗组血清中TGFβ1的表达增强,与对照组相比有明显差异(P<0.01)。③股骨头局部TGFβ1mRNA的转录:治疗组在第6周时表达即增强,在第8周时表达又降低,而对照组在第6周时表达减少,在第8周时未检测到TGFβ1mRNA的表达,两组有明显差异(P<0.05,P<0.01)。结论:活血化瘀中药可促进激素性股骨头缺血坏死模鼠股骨头局部TGFβ1mRNA的转录水平以及股骨头局部TGFβ1的表达,促进坏死股骨头的修复。更多还原

【Abstract】 Objective:To establish rat models of Steroid-avascular necrosis of femoral head,observe the effects of activating blood circulation of chinese herbal medicine on genetic expression of transforming growth factor-β1(TGF-β1).To interpret the mechanism of the effect on Steroid-avascular necrosis of femoral head by activating blood circulation,and offer a effective method to clinical.Methods:Cleaner-40 SD rats,half males and half females,weight(200±20) g,were randomly divided into 2 groups:4 rats were in common group and 36 rats were in medel group.The rats in medel group were administered with 24.5 mg/kg hydroxyprednisone twice a week peritoneal injection for 6 weeks induced to femur head necrosis.The rats in common group were through gluteus injection as control.There were 4 rats were killed in each group after 6 weeks,to be assure that the model were succed.All surplus rats were divided into treatment group and control group:the treatment group were administrated with activating blood circulation of Chinese herbal medicine 12.3 ml/kg per day,the control group were administrated with sodium chloride 12.3 ml/kg per day.Then,after 6 and 8 weeks,killed the animal and detected all indexes.Results:1.The expression of TGF-β by immunohistochemistry and image analysis:the expression of femoral head TGF-β increased significantly in treatment group than in control group and two group had significant differences.2.Serum levels of TGF-β1:in the treatment group serum expression of TGF-β1 increased,compared with the control group had significant difference(P<0.01).3.The femoral head local TGF-β1 mRNA transcription:treatment group in the first six weeks,expressed that the increase in the first eight weeks,expressed also reduced,and the control group in the first six weeks,expressed a decrease in the first eight weeks,was not detected to TGF-β1 mRNA expression of difference between the two groups was significant(P<0.01).TGF-β1 mRNA in serum and the femoral head with local expression of TGF-β1 in consistency.Conclusion:Rat abdominal cavity prednisolone acetate injection plus intermittent standing avascular necrosis modeling stability,good repeatability.Taohongsiwu through the promotion of hormone-ischemic necrosis of the femoral head rat model of TGF-β1 mRNA transcription,and promote expression of TGF-β1.更多还原