【摘要】 以紫花苜蓿品种阿尔冈金为受体材料,以含gus基因的植物双元表达载体pPZP221为外源基因供体进行了花粉管通道法转基因研究。于授粉后6h、9h、12h、20h、24h、28h、32h、48h滴加质粒DNA溶液,荚果成熟后收获转化种子,分别使用2套引物对T0代植株进行PCR检测,结果表明:20~48h所得到的T0代植株均具有较高的转化频率,其中授粉后32h导入质粒DNA所获得的转化率最高,为16.7%;PCR检测初步证明外源基因已整合到受体植物基因组中。更多还原

【Abstract】 In order to establish a simple,practical technique with high transformation frequency in alfalfa(Medicago sativa L.),the pollen-tube pathway method was used for alfalfa transformation.The alfalfa cultivar ’Algonquin’ was used as a recipient and the plant binary vector pPZP221 containing gus gene was used as donor DNA.The plasmid DNA solution was applied to the stigma at 6h,9h,12h,20h,24h,28h,32h and 48h after hand-pollination,respectively.PCR results showed that the transformation rate of T0 seedlings from 20h to 48h was relatively high and the highest rate was up to 16.7% at 32h after pollination.This results suggested that foreign DNA was inserted into the genome of transgenic T0 seedlings.更多还原