【摘要】 目的:探讨儿科呼吸系统感染大肠埃希菌临床分离株耐药性与耐药质粒相关性,指导临床合理用药。方法:采用常规方法分离鉴定北京地区儿科呼吸道感染大肠埃希菌,纸片琼脂扩散法进行药敏试验,碱裂解法抽提质粒。结果:45株大肠埃希菌中25株为多重耐药,对氨苄两林、头孢呋辛、头孢唑啉的耐药率达100%,对头孢噻肟、头孢曲松、氨曲南、氨基糖苷类、复方新诺明及非β-内酰胺类抗菌药物的耐药率高于56%,并携带3～6个数目不等的大小约为1.2～60kb的天然质粒。结论:北京地区儿科呼吸道感染大肠埃希菌的耐药率高,其耐药性与细菌携带1.2～60kb的质粒密切相关。更多还原

【Abstract】 Objective:To explore the correlation between drug resistance and resistant plasmid in 45 strains of Escherichia coli in pediatric respiratory system infection,and direct the rational administration.Methods:Escherichia coli were isolated and identified by routine methods from the infected cases of pediatric respiratory system in Beijing.Agar diffusion method was applied to susceptibility test and plasmids were extracted by alkaline lysis.Results:Among 45 strains of Escherichia coli,25 strains were multiple drug resistant.The resistance rates to ampicillin,cefuroxime and cefazolin were 100%,while the resistance rates to cefotaxime,ceftriaxone,aztreonam,aminoglycosides, TMP-SMZ and non-β-lactam antibiotics were 56%.The 25 drug resistant strains carried about 3～6 natural plasmids in size from 1.2 to 60 kb.Conclusion:The resistance rate of Escherichia coli in pediatric respiratory system infection is high in Beijing,which is related to resistant plasmids in size from 1.2 to 60 kb closely.更多还原