【摘要】 目的:探讨导流杂交法人乳头瘤病毒(HPV)基因型检测在宫颈疾病病因学诊断中的应用价值。方法:采用导流杂交法对236例宫颈疾病筛查者(包括宫颈癌组31例、宫颈上皮内瘤变组10例、炎症组118例和正常组77例)宫颈脱落细胞进行21种HPV基因型检测,分析各组患者不同HPV亚型感染特点。结果:从236例妇女中共检出HPV(+)者72例,包括宫颈癌组29例(93.5%)、宫颈上皮内瘤变组5例(50.0%)、炎症组30例(25.4%)、正常组8例(10.4%),各组阳性率比较差异有统计学意义(χ2=76.041,P<0.001);HPV基因型检出率由高到低依次为:HPV-16(33.3%)、HPV-52(16.1%)、HPV-58(8.0%)、HPV-31(8.0%)、HPV-18(6.9%)、HPV-11(6.9%)、CP8304(3.4%)、HPV-33(3.4%)、HPV-53(2.3%)、HPV-59(2.3%)、HPV-68(2.3%)、HPV-66(2.3%)、HPV-6(1.1%)、HPV-39(1.1%)、HPV-56(1.1%)、HPV-42(1.1%)、HPV-43(1.1%)、HPV-45(1.1%);高危HPV基因型检出率随宫颈病变程度加重而明显升高(χ2=61.190,P<0.001),各疾病组检出的前3位HPV亚型均为高危型。结论:宫颈疾病与HPV感染密切相关,宫颈肿瘤主要由高危基因型HPV感染所致;导流杂交法具有操作简捷、结果清晰和比较经济等优势,值得在宫颈疾病的病因诊断中推广应用。更多还原

【Abstract】 Objective:To evaluate the clinical application value of flow-through hybridization in identifying of human papillomavirus(HPV) genotypes for etiological diagnosis of cervical diseases.Methods:236 cases of local women were subdivided into four groups according to their histological diagnosis(31 cases of cervical cancer,10 cases of cervical intraepithelial neoplasia(CIN),118 cases of chronic cervicitis,and 77 cases of normal histological manifestation).Cervical cells prepared from 236 cases were used to detect the HPV genotypes by flow-through hybridization and the composition and distribution pattern of HPV genotypes within four group were analyzed.Results:Among 236 cases of samples,72 cases were detected to have HPV infection,from which the positive cases in cervical cancer,CIN,chronic cervicitis,and normal groups were 29(93.5%),5(50.0%),30(25.4%)and 8(10.4%),respectively.The detection rate of HPV infection among four groups were significantly different(χ2=76.041,P<0.001).The percent distribution of detecting HPV genotypes were HPV-16(33.3%),HPV-52(16.1%),HPV-58(8.0%),HPV-31(8.0%),HPV-18(6.9%),HPV-11(6.9%),CP8304(3.4%),HPV-33(3.4%),HPV-53(2.3%),HPV-59(2.3%),HPV-68(2.3%),HPV-66(2.3%),HPV-6(1.1%),HPV-39(1.1%),HPV-56(1.1%),HPV-42(1.1%),HPV-43(1.1%),HPV-45(1.1%).The ratio of high-risk HPV genotypes was increased with aggravation of cervical lesion(χ2=61.190,P<0.001).The 3 most common genotypes of HPV in the patients’ groups were all high-risk HPV genotypes.Conclusion:Cervical diseases have close relationship to HPV infection and cervical carcinomas are mainly resulted from high-risk HPV genotypes’ infection.Flow-through hybridization can become an ideal approach to determining the HPV genotypes and should be promoted for use during etiological diagnosis of cervical diseases.更多还原