【摘要】 目的:探讨微粒体环氧化物水解酶(EPHX)基因多态性与中国妇女多囊卵巢综合征(PCOS)发病易感性的关系。方法:用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析的方法检测115例PCOS病人和89例健康对照者EPHX基因外显子3(Tyr113→His)、外显子4(His139→yArg)基因型分布。结果:PCOS组和对照组基因型分布未见明显异常。两组外显子3和外显子4的基因型分布无统计学差异,P=0.185(exon3)和P=0.205(exon4);单点等位基因的分布也未见显著差别P=0.135(exon3)和P=0.135(exon4)。但对比预测的高、中、低和极低酶活性时发现高酶活性的基因突变相对于其他酶活性在PCOS疾病的发生上可以视为一种保护因素(OR=0.605,CI=0.158～2.324)。结论:由于基因变异导致的EPHX高活性基因型可能在多囊卵巢综合征的发病中起保护作用。更多还原

【Abstract】 Objective:To explore the relationship between susceptibility of polycystic ovary syndrome (PCOS) and gene polymorphism of microsomal epoxide hydrolase (EPHX) in chinese women.Methods:Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were performed to detect genotype EPHX polymorphism in exon3 (Tyr113→His) and exon4 (His139→Arg) from 115 PCOS women (case group) and 89 healthy women (control group).Results:There was no significant difference in the distribution of genotypes in EPHX between case group and control group . Genotype distributions of exon 3 and exon 4 had no significant difference between two groups P=0.185 (exon3)P=0.205 (exon4).There was no significant difference in the distribution of single point allele between two groups P=0.135 (exon3)P=0.135 (exon4).There was positive correlation between genic mutation of high-activity EPHX and PCOS (OR=0.605,CI=0.158～2.324).Conclusion:Genetype of high-activity EPHX induced by genic mutation may play a role in the protection of PCOS.更多还原