【摘要】 目的阐述MMP-2、MMP-9与胶质瘤的浸润机制。方法应用免疫组化方法和原位杂交方法检测胶质瘤和胶质瘤浸润组织中的MMP-2、MMP-9蛋白及MMP-2 mRNA、MMP-9 mRNA的表达。结果MMP-2 mRNA和MMP-9 mRNA表达在Ⅰ、Ⅱ、Ⅲ和Ⅳ级胶质瘤的阳性表达分别为5.26±1.47、9.51±0.96、14.58±1.42、20.56±1.56和7.90±0.50、14.46±0.75、23.54±1.32、28.07±0.81。MMP-2 mRNA和MMP-9 mRNA在Ⅰ～Ⅱ级胶质瘤浸润组织与Ⅲ～Ⅳ级胶质瘤浸润组织中的阳性表达分别为5.70±0.95、8.20±0.43和2.30±0.14、10.32±0.65。两者之间有显著差异(P<0.01)。结论胶质瘤的恶性程度与MMP-2、MMP-9表达呈正相关,随着胶质瘤的恶性程度的增高而增加。恶性程度高的胶质瘤,分泌基质金属蛋白酶能力越强,肿瘤的浸润性也越强。在胶质瘤治疗的过程中,即使肿瘤组织自身被切除,浸润组织则是胶质瘤复发的根源。更多还原

【Abstract】 Objective To discusse the expression of MMP-2 and MMP-9 in human glioma and glioma’s invasiveness and to elucidate invasive mechanism.Methods The protein expression of MMP-2 and MMP-9 was detected through immunohistochemistry,and MMP-2 mRNA and MMP-9 mRNA were detected through in situ hybridization in glioma and glioma’s invasive tissue.Results The positive expression of MMP-2 mRNA and MMP-9 mRNA in glioma was 5.26±1.47,9.51±0.96,14.58±1.42,20.56±1.56 and 7.90±0.50,14.46±0.75,23.54±1.32,28.07±0.81,respectively.The expression of MMP-2 mRNA and MMP-9 mRNA inⅠ~Ⅱgrade and Ⅲ~Ⅳgrade was 5.70±0.95,8.20±0.43 and 2.30±0.14,10.32±0.65,respecttively.Conclusions There is positive correlation with the malignant degree of glioma and the expression of MMP-2 and MMP-9.The glioma with high malignancy,comparing with low malignancy,shows stronger invasiveness.The more the expressions of MMP-2 and MMP-9 in glioma is,the stronger the invasive capacity is.The glioma’s invasive tissue is the recurring source of glioma in clinical treatment,even after resecting glioma.更多还原