【摘要】 目的探讨川芎嗪对MPTP所致小鼠多巴胺能神经元损伤的保护作用及可能机制。方法C57BL/6J雄性小鼠32只,随机分为4组:生理盐水对照组(NS+NS)、生理盐水组(NS+MPTP)、川芎嗪高剂量组(LT50+MPTP)、川芎嗪低剂量组(LT20+MPTP),每组8只动物。分别采用HPLC法检测纹状体中DA的含量,免疫组化检测黑质中TH阳性细胞数,荧光显色法检测黑质SOD活力、GSH含量。结果LT50+MPTP组纹状体DA含量、黑质DA神经元数量、黑质SOD活力、黑质GSH含量较NS+MPTP组显著增高(P<0.01)。结论川芎嗪对对MPTP所致的小鼠多巴胺能神经元损伤具有保护作用,其保护机制可能与其调节调节小鼠黑质中的SOD、GSH含量有关。更多还原

【Abstract】 Objective To investigate the neuroprotective effects and mechanisms of ligustrazine on the MPTP-induced dopaminergic neurodegeneration in a mouse model of PD.Methods Male C57BL/6J mice were randomly divided into following four treatment groups(n=8/group):(1)the saline control group(NS+NS),mice pretreated with saline followed by saline treatment;(2)the mice pre-treated with saline followed by saline treatment(NS+ MPTP);(3)the mice pretreated with 50mg/kg of ligustrazine followed by MPTP treatment(LT50+MPTP);(4)the mice pretreated with 20mg/kg of ligustrazine followed by MPTP(LT20+MPTP).HPLC,immunohistochemistry and fluorimetry were used.Results The residual DA contents,TH-IR positive cells,SOD activity and GSH content in the mice pretreated with 50mg/kg ligustrazine(LT50+MPTP)were significantly higher than those of the saline-pretreatd mice(NS+MPTP)(P<0.01).Conclusions Ligustrazine ameliorated MPTP-induced dopaminergic neurodegeneration in mice.The neuroprotective effect of ligustrazine may be associated with their strong antioxidant capacity in vivo.更多还原