【摘要】 目的探讨血红素加氧酶-1基因启动子区域GT重复序列[HO-1(GT)n]遗传多态性,神经元特异性烯醇化酶(NSE)与脑梗死的遗传易感性的关系。方法采用巢式PCR法检测HO-1(GT)n,酶联免疫吸附法(ELISA)检测血清NSE,分析55例急性脑梗死(ACI组)患者和56例对照者的外周血样本。结果ACI组短GT重复序列分布43.64%明显低于对照组64.29%,差别有统计学意义。在急性脑梗死患者治疗前后均显示短GT重复序列组NSE水平低于长GT重复序列组NSE水平。结论HO-1基因启动子短重复序列脑梗死患者血清NSE水平较低,HO-1短GT重复序列遗传多态性可能与脑梗死的遗传易感性有关。更多还原

【Abstract】 Objective To investigate the interrelation between genetic polymorphism of Heme oxygenase-1 genic repeated sequence in promoter region(HO-1(GT)n)and serum levels of neuron-specific enolase(NSE)in hereditary susceptibility of cerebral infarction.Methods The information and peripheral blood sample came from 55 acute cerebral infarction(ACI group)and 56 healthy people as control group were collected.HO-1(GT)n was detected by PCR,and NSE was detected by ELISA.Results The number of HO-1 genotype with S allele was significantly different between the ACI group(43.64%)and control group(65.45%).The level of serum NSE before treatment in ACI-group was significantly different with that of after treatment ACI-group and control group(P<0.05).Conclusion The ACI patients whose HO-1 genotype with S allele have lower level of serum NSE than the ACI patients whose HO-1 genotype without S allele,and neuron was less damaged.The less repetitive sequence exhibits a reduced risk for ACI.更多还原