【摘要】 目的探讨脑出血患者脑组织中NF-кB(nuclear factor-kappaB)的表达特征及意义。方法筛选临床脑出血患者死亡病例21例,按出血时间(发病至死亡时间)分为6组:0~6h、6~12h、12~24h、24~48h、48~72h、72~96h。自出血灶边缘13cm处、海马和大脑皮层及出血灶对侧相应部位的脑组织取材,出血灶对侧设为对照组。采用免疫组织化学染色观察NF-кB在脑组织中的表达。结果脑出血后6h时出血灶周围即有NF-кB蛋白表达(5.27±0.12),6~12h表达增加(8.52±0.66),24~48h时达高峰(34.47±2.81),72~96h表达减少(19.50±2.69),与对照组比较差异无统计学意义(P>0.05),但与0~6h组比较差异有统计学意义(P<0.05)。NF-кB蛋白还表达于两侧海马和大脑皮层。结论脑出血后脑组织中NF-кB表达增加,对脑水肿的形成可能有一定的促进作用。更多还原

【Abstract】 Objective To study the expression and significance of NF-кB in human brain after intracerebral hemorrhage.Method 21 human clinical fatal cases with ICH were divided into 6 groups according to different onset time:0 6h,6 12h,12 24h,24 48h,48 72h and 72 96h group.The brain tissues were removed frome 1 3cm away from the margin of the hemorrhagic lesion,hippocampus and cerebral cortex of the side of hematoma,as well as tissues from the corresponding areas of other side were taken as controls.NF-кB expression was detected by immunohistochemistry.Result There was little expression of NF-кB at 6h after ICH(5.27±0.12).The expression of NF-кB begun to increase at 6~12h(8.52±0.66),reached the peak at 24~48h(34.47±2.81),and gradually decreased at 72~96h(19.5±2.69).There had no significant difference compared with normal contralateral regions(P>0.05),but had significant difference compared with 0~6h group(P<0.05).There was expression of NF-кB in hippocampus and cortex.Conclusion The increased expression of NF-KB after intracerebral hemorrhage is probably correlated with the brain edema formation in the brain tissues around the hematoma.更多还原