【摘要】 目的观察丹参素异丙酯的舒张血管作用,并探讨其作用机制。方法以大鼠肺动脉为标本,采用离体血管灌流方法,观察丹参素异丙酯对去甲肾上腺素(NE)预收缩动脉的舒张作用以及血管内皮细胞、血管平滑肌在该效应中的作用。结果丹参素异丙酯可浓度依赖性舒张NE预收缩的完整内皮血管,去内皮后该作用明显降低。可使KCl量效曲线右移,并能浓度依赖地抑制NE诱导的内钙释放和经受体操纵性钙通道(ROCC)的外钙内流。L-Nω-硝基精氨酸甲酯(L-NAME)、吲哚美辛以及CsCl、四乙铵、格列本脲和BaCl2均可显著减弱丹参素异丙酯的舒血管作用,而普萘洛尔、美托洛尔及沙丁胺醇对丹参素异丙酯的舒血管效应无明显影响。结论丹参素异丙酯可内皮依赖性地舒张大鼠肺动脉,其舒张血管效应可能与NO、前列环素(PGI2)途径以及钙通道、钾通道有关。更多还原

【Abstract】 Objective To investigate the relaxant effect of salanic acid A isopropyl ester(isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate)and its mechanism.Methods Isolated rat pulmonary artery was perfused and the tension of the vessel was measured.To observe the relaxant effect of salianic acid A isopropyl ester on the pulmonary artery precontracted by noradrenaline(NE)and the role of endothelial cell and vascular smooth muscle cell on vasodilation.Results Salianic acid A isopropyl ester relaxed the endothelium-intact artery precontracted by NE in a concentration-dependent manner,and the effect was depressed with denuded endothelium.Salianic acid A isopropyl ester right-shifted the concentration-response curve of KCl and suppressed the contractions induced by NE and CaCl2 in the Ca2+-free-K-H solution.The relaxant effect of salianic acid A isopropyl ester was inhibited by Nω-nitro-L-arginine methyl ester hydrochloride(L-NAME),Indometacin,CsCl,Tetraethylammonium(TEA),Glibenclamide,and BaCl2,but not that by Propranolol,Metoprolol,and Salbutamol.Conclusion Salranic acid A isopropyl ester could significantly relax the pulmonary artery of rats,which may derive in part from endothelium.The relaxant effect of salianic acid A ispropyl ester may relate to NO pathway,prostacyclin(PGI2)pathway,calcium channel,and potassium channel.更多还原