【摘要】 目的:建立一种反相高效液相色谱方法,同时测定刺五加脑灵液中两种有效成分的含量。方法:采用Alltech C18柱,甲醇(A)-0.4%冰醋酸(B)溶液梯度洗脱;检测波长切换法:异嗪皮啶344 nm,五味子醇甲254 nm。结果:线性关系良好,线性范围分别为:0.88~17.6μg/mL,0.76~15.2μg/mL,回收率异嗪皮啶为100.64%,RSD为1.07%;五味子醇甲为100.33%,RSD为0.65%。结论:本测定方法简便可行、重复性好,可用于制剂的质量控制。更多还原

【Abstract】 AIM: To establish a method of simultaneously determining effective components in Ciwujia Naoling Oral Liquid by RP-HPLC. METHODS: Stationary phase: Alltech C₁₈ column,the mobile phase was methanol（A）-0.4% HAC-water（B） with linear gradient elution.Detection was performed at wavelength of 344 nm,254 nm respectively for isofraxidin and schisandrin. RESULTS: The two effective components could be detected by this method,and their contents could be determined.A good linearity of isofraxidin and schisandrin was in the range of 0.88-17.6 μg/mL,0.76-15.2 μg/mL respectively.The average recoveries were 100.64%,100.33% respectively,RSD were 1.07%,0.65% respectively. CONCLUSION: The method is simple,feasible and reproducible and can be used for the quality control of Ciwujia Naoling Oral Liquid.更多还原