【摘要】 目的:采用HPLC梯度洗脱法同时测定柴丹解郁颗粒中丹参酮ⅡA和丹酚酸B的含量。方法:用Hypersil-ODS C18色谱柱(150 mm×4.6 mm,5μm);甲醇:水梯度洗脱:0~20 min(35~90:65~10)、20~25 min(90~95:10~5)、25~30min(95:5);柱温:30℃;检测波长:0~20 min 282 nm、20~30 min 270 nm;流速:1.0 mL/min。结果:丹参酮ⅡA、丹酚酸B的线性范围分别是0.014448~0.09632μg(r=0.9999)、0.1986~3.972μg(r=0.9999),平均回收率分别为99.62%(RSD为2.28%)、100.58%(RSD为2.50%)。结论:本方法准确可靠,专属性强,结果稳定,重现性好,可较好的控制柴丹解郁颗粒的质量。更多还原

【Abstract】 AIM: To determine tanshinone Ⅱ_A and salvianolic acid B by HPLC gradient elution. METHODS: HPLC was used with Hypersil-ODS C₁₈ column（150 mm×4.6 mm,5 μm）.The mobile phase consisted of methanol-water 0²0 min（35-90:6510）,20-25 min（90-95:10-5）,25-30 min（95:5）gradient elution.The flow rate was 1.0 mL/min with column temperature at 30 ℃.The UV detection wavelength was at 286 nm in 0-20 min and 270 nm in 20-30 min,respectively. RESULTS: The linear range of tanshinone Ⅱ_A and salvianolic acid B were in the range of 0.014 448-0.096 32（r=0.999 97）, and 0.198 6-3.972 μg（r=0.999 94）,respectively.The average recoveries were 99.62%（RSD=2.28%） and 100.58%（RSD=2.5%）,respectively.（CONCLUSION:） The method is accurate、sensitive、reproducible,and suitable for the quality control of Chaidan Jieyu Granules.更多还原