【摘要】 目的:探讨血管内皮生长因子(VEGF)转染的神经干细胞(NSC)对放射性脑损伤的影响。方法:将Sprague-Dawley(SD)大鼠随机分为对照组、放射性脑损伤组、NSC和转染VEGF的NSC治疗组,放射性脑病模型成功制备后1周脑内移植转染VEGF的NSC,移植后1周、2周、3周、4周和6周末取其脑组织,用免疫组织化学法测定神经元特异性烯醇化酶(NSE)阳性细胞数,Western blotting法检测NSE蛋白表达情况。结果:与对照组相比,放射性脑损伤组大鼠脑组织中NSE阳性细胞数显著下降(P<0.05);与放射性脑损伤组相比,NSC治疗组和转染VEGF的NSC治疗组在移植3周后NSE阳性细胞数明显增加(P<0.05),与NSC治疗组比较,在移植6周末转染VEGF的NSC治疗组NSE的阳性细胞数增加更明显(P<0.05)。与放射性脑损伤组比,NSC治疗组和转染VEGF的NSC治疗组在移植3周末NSE蛋白表达量明显增加(P<0.05),与NSC治疗组比较,在移植6周末转染VEGF的NSC组NSE蛋白表达量增加更明显(P<0.05)。结论:转染VEGF的NSC移植可提高放射性脑损伤组织中NSE的含量。更多还原

【Abstract】 AIM:To explore the effect of neural stem cells(NSC) transfected with vascular endothelial growth factor(VEGF) on brain injury irradiated by radioaction.METHODS:Mature Sprague-Dawley(SD) rats were randomly divided into 4 groups:control group,radiation brain injury group,NSC therapeutic group,NSC transfected with VEGF(NSC/VEGF) therapeutic group.NSC transfected with VEGF was transplanted into the rat brain of therapeutic group after preparation of radiation encephalopathy model for 1 week.The brain tissue was taken at week 1,2,3,4 and 6 after transplantation.Immunohistochemical method was used to assay the count of neuro-specific enolase(NSE) stained cells in brain tissue.Western blotting was used to detect the expression of NSE protein.RESULTS:Compared to control group,the count of NSE stained cells in brain of radiation brain injury group was decreased significantly(P<0.05).Compared to that in radiation brain injury group,the count of NSE stained cells in brains was enhanced in NSC therapeutic group and NSC/VEGF therapeutic group since 3 weeks after transplantation(P<0.05).Compared to that in NSC therapeutic group,the count of NSE stained cells in NSC/VEGF therapeutic group was more enhanced since 6 weeks after transplantation(P<0.05).Compared to that in radiation brain injury group,the expression of NSE protein was enhanced in NSC group and NSC/VEGF group since 3 weeks after transplantation(P<0.05).Compared to that in NSC group,the expression of NSE protein in NSC/VEGF group was more enhanced since 6 weeks after transplantation(P<0.05).CONCLUSION:Transplantation of NSC transfected with VEGF may increase the content of NSE in rat brain of radiation encephalopathy model.更多还原