【摘要】 目的:建立小鼠矽肺动物模型,探讨姜黄素在减轻小鼠矽肺纤维化中的作用。方法:用手术方法暴露小鼠气管,定量注射二氧化硅(SiO2)悬液建立矽肺模型。建模2周后将小鼠随机分为模型组、低剂量组、中剂量组和高剂量组分别给药,给药2周以及6周后处死动物,对肺组织行病理组织学检查。结果:建立了矽肺的纤维化模型。与模型组相比,给药2周后,高剂量组矽结节大小及数量显著减小(P<0.05);给药6周后,模型组以成纤维细胞为主,同时夹杂少量的胶原纤维,而给药组仍以巨噬细胞和淋巴细胞为主的细胞性矽结节为主。结论:姜黄素能抑制矽肺纤维化进程。更多还原

【Abstract】 AIM:To establish an animal model and to investigate the effect of curcumin on SiO2-induced pulmonary fibrosis in mice.METHODS:Animal model was established by direct tracheal instillation of silica into rat lungs through tracheostomy.Two weeks after tracheostomy these mice were divided into four groups,which were poured into curcumin at different concentrations(high,medium,low) through stomach.In control group,distilled water is used instead of curcumin.After 2 weeks and 6 weeks,the animals were killed and histopathological test was performed on lung.RESULTS:The silicosis model was established.Two weeks after treatment,the numbers and size of the nodules in high dosage group were much less and smaller than those in control group(P<0.05).Six weeks after treatment,the nodules in control group were mainly consisted of fibroblast mixed with a small amount of collagen fiber,whereas nodules in experimental group were made up of macrophages.CONCLUSION:Curcumin inhibits the process of silicotic fibrosis.更多还原