【摘要】 目的:探讨Graves病(GD)患者外周血单个核细胞(PBMCs)NF-кB活性变化及其在GD免疫病理机制中的意义。方法:分别采集22例未经治疗的GD患者(未经治疗组)、20例经他巴唑治疗>1年的GD患者(治疗组)及25例健康志愿者(对照组)静脉血,采用凝胶电泳可动性移位分析(EMSA)检测PBMCs中NF-кB活性;采用放免法检测血清IL-1β、IL-6及TNF-α含量。结果:未经治疗GD组患者PBMCs NF-кB活性显著高于治疗组及正常对照组(P<0.05);未经治疗GD组患者血清IL-1β、IL-6、TNF-α水平明显高于治疗组和正常对照组(P<0.05);未经治疗GD组和治疗组NF-кB活性与IL-6水平均呈正相关。结论:GD患者NF-кB活性明显升高,NF-кB活性增高可能在GD免疫病理机制中起重要作用。更多还原

【Abstract】 AIM:To investigate the activity of NF-кB in peripheral blood mononuclear cells (PBMCs) from patients with Graves disease (GD) and the significance in immunopathogenesis of GD.METHODS:Peripheral blood was collected from 22 untreated GD,20 treated GD with tapazole more than 1 year,and 25 healthy volunteers. PBMCs were isolated from the blood by histopaque-1077 density-gradient centrifugation. The activity of NF-кB in PBMCs was analyzed using gel electrophoretic mobility shift assay (EMSA). The contents of IL-1β,IL-6 and TNF-α were tested by radioimmunoassay.RESULTS:The activity of NF-кB in PBMCs of untreated GD group was increased remarkably,compared with that in the treated group and control (P<0.05). The contents of IL-1β,IL-6 and TNF-α in untreated group were significantly higher than those in treated GD and control group (P<0.05). A positive correlation between NF-кB activity and IL-6 level in untreated GD group and treated GD group was observed.CONCLUSION:The activity of NF-кB in PBMCs with GD patients is increased significantly,which might play an important role in the immunopathogenesis of GD.更多还原