【摘要】 目的研究姜黄素抑制人黑素瘤A375细胞增殖及诱导凋亡的作用。方法体外培养人黑素瘤A375细胞,倒置显微镜观察细胞形态;采用MTT法检测细胞增殖抑制作用;Annexin V/PI双染法检测细胞凋亡。结果姜黄素可使A375细胞体积缩小,细胞变圆;姜黄素对人A375细胞的生长增殖有明显的抑制作用,并且这种作用呈时间和剂量依赖性;Annexin V/PI双染法证实姜黄素可以诱导A375细胞发生凋亡。结论姜黄素对人黑素瘤A375细胞具有抑制增殖和诱导凋亡作用。更多还原

【Abstract】 Objective To study the effect of curcumin on proliferation,inhibition,and apoptosis in human melanoma A375 cells.Methods After human melanoma A375 cells were treated with various concentrations of curcumin,the morphological alterations of A375 cells were observed by inverse microscopy.The inhibition of cell proliferation was measured by MTT assay.Apoptosis rate was analyzed by flow cytometry.Results A375 cells grew round and small obviously after being treated with curcumin.Proliferation of A375 cells was significantly inhibited by curcumin in a time-and dose-dependent manner(P<0.01).Fluorescent staining can detect the apoptotic cells.Conclusion Curcumin could inhibit proliferation and induce apoptosis of human melanoma A375 cells in vitro.更多还原