【摘要】 目的分析SLE患者GATA-3、MBD2与IL-4表达的相关性,探讨GATA-3和MBD2对IL-v4表达的调控作用。方法应用实时定量PCR法检测35例SLE患者和20例正常人外周血单核细胞(PBMC)中IL-4、GATA-3、MBD2的mRNA表达水平,并分析它们之间的相关性。结果SLE患者外周血单核细胞中IL-4、GATA-3和MBD2的表达水平均明显高于正常对照组(t=3.37,3.55,9.68,P均<0.05);IL-4与GATA-3、IL-4与MBD2的表达水平均呈正相关(r=0.39,0.97,P均<0.05)。结论SLE患者中IL-4表达水平升高可能是由于MBD2对IL-4基因阻遏作用降低,及大量GATA-3对IL-4基因的过度激活共同作用所导致。更多还原

【Abstract】 Objective To analyze correlation between GATA-3,MBD2 and to IL-4 gene expression, and to investigate the effect of GATA3 and MBD2 on IL-4 gene expression in SLE patients. Methods The transcription levels of IL-4,GATA-3,MBD2 were detected by using RT-qPCR and correlations were conducted by correlation analysis using spss 11.5 software. Results The expression levels of IL-4,GATA-3 and MBD2 in SLE patients were significantly increased, and there was statistical difference between patients with SLE and normal controls(t=3.37, 3.55, 9.68, all P<0.05,).There was positive correlation between IL-4 and GATA-3, IL-4 and MBD2 (r=0.39,0.97, all P<0.05). Conclusion The high expression levels of IL-4 may be caused by loss of gene-slience function of MBD2 and excessive activation of GATA-3 in SLE patients.更多还原