【摘要】 在离体培养条件下比较了秋水仙素不同浓度、不同处理时间诱导杂种春石斛体细胞染色体加倍的效果。结果表明,0.06%秋水仙素处理12h的诱变效果最佳,诱变率达69.1%。用组织培养结合不定芽诱导技术获得了稳定的多倍体变异株。变异株与正常株形态差异明显,气孔数及大小之间差异显著。二倍体对照2n=2x=38,变异株2n=4x=76(四倍体),同时也发现存在嵌合体现象。更多还原

【Abstract】 A hybrid of nobile-type Dendrobium was treated with colchicines at different concentration and time duration in vitro.The results showed that the best effect was obtained by the treatment of 0.06% colchicines with 12 hours and its induction rate was 69.1%. Comparing the characteristics of plants and stomas,there were remarkable difference between the treatment and the control. Chromosome observation found that the chromosome number of treated plants was 76 and the chromosome number of the control was 38,indicating the tetraploid(2n=4x=76) of nobile-type Dendrobium was induced successfully.The mixer-fold phenomenon was also found among treatment plants.更多还原