【摘要】 在非洲菊(Gerbera hybrida)舌状花离体培养条件下,研究了外源NH4+对花瓣着色与生长的影响。结果表明,10.00mmol·L-1NH4+明显抑制舌状花花瓣的着色与展开,宽度、鲜样质量和干样质量也明显下降。对照(3%蔗糖溶液)舌状花花色素苷含量在36h后开始缓慢增加,54～84h快速积累,以后增加较慢。在36h之内进行NH4+处理,可完全抑制着色与展开,而36h之后进行处理则抑制作用减弱。NH4+处理时间长于12h,则抑制作用更加明显。NH4+处理下,蔗糖含量的增加能够促进花色素苷的积累,果糖、乳糖、麦芽糖和葡萄糖等不影响NH4+对着色的抑制作用。更多还原

【Abstract】 Ray floret (rf) petals detached from inflorescences of Gerbera hybrida were cultured in vitro and the effects of exogenous ammonium on petal pigmentation and growth were investigated. NH4+ of 10.00 mmol·L-1 obviously blocked petal pigmentation and the petal expansion, the width, fresh weight and dry weight were also declined significantly. The anthocyanin content of the petals of ray floret cultured in vitro began to increase 36 h and accumulated greatly fast between 54 h and 84 h after the incubation, which was followed by a gradually decline. The application of NH4+ within 36 h was completely effective on the inhibition of the pigmentation, while the effect was gradually decreased when NH4+ was applied after 36 h. Moreover, stronger inhibition was observed when the working time of the NH4+ was longer than 12 h. The anthocyanin accumulation was promoted by the increase of sucrose content in the present of NH4+. Other sugars such as fructose, lactose, maltose and glucose in the media had little effect on the NH4+-induced inhibition of pigmentation.更多还原