【摘要】 利用大白菜细胞核复等位基因型雄性不育两用系‘AB01’的不育株,与多国芸薹属植物基因组计划MBGP的基础材料‘Chiifu’杂交及回交,构建BC1群体。根据SSR检测体系中各主要成份浓度对扩增结果的影响,优化反应体系。选用250对SSR引物,对大白菜核基因雄性不育复等位基因Ms进行SSR+BSA分析,获得了8对多态性引物。在101株BC1个体间对这些引物进一步检测,得到2个与Ms基因连锁的SSR标记cnu_m273和cnu_m295。经连锁分析,二者位于Ms的同一侧,遗传距离分别为4.95cM和7.92cM。更多还原

【Abstract】 A BC1 mapping population was constructed with Chinese cabbage inbred line’Chiifu’ which is the model material of Multinational Brassica Genome Project as the male parent and genic multiple allele male sterile material ’AB01’ as the female parent.Major effects of components in SSR-PCR system on amplification result were probed.250 SSR primer pairs were employed to identify SSR markers linked to the genic multiple allele male sterile gene ’Ms’.Within the eight SSR primer pairs showing polymorphism between two gene bulks,two SSR markers named cnu_m273 and cnu_m295 were found linked to the Ms gene,at the genetic distances of 4.95 cM and 7.92 cM respectively.更多还原