【摘要】 针对饮用水中消毒副产物与微囊藻毒素的低剂量复合污染问题,采用离体细胞培养诱导方法,以草鱼淋巴细胞为暴露受体,研究了MCLR、MCRR两种微囊藻毒素和CHClBr2、CHCl2Br两种消毒副产物的单独与联合毒效应.结果表明:4种污染物在试验浓度下暴露2h即能使草鱼淋巴细胞发生凋亡,且呈显著的剂量-效应关系;1nmol·L-1MCLR、MCRR分别和1～100nmol·L-1的CHCl2Br、CHClBr2的联合作用均表现为相加作用,并且均呈显著的剂量-效应关系.草鱼淋巴细胞凋亡率可作为一种有效的指标来评价微囊藻毒素及消毒副产物复合污染引起的细胞毒性.更多还原

【Abstract】 Aiming at the combined pollution of low dose microcystins (MCs) and disinfection by-products (DBPs) in drinking water,the individual and combined cytotoxic effects of two MCs (MCLR and MCRR) and two DBP compounds (CHClBr2 and CHCl2Br) on the apoptosis of isolated grass carp (Ctenopharngodon idellus) lymphocytes were examined by using in vitro bioassays. The results showed that after 2 hours exposure to the four pollutants within the range of test concentrations,the apoptosis of isolated lymphocyte occurred,and there existed significant dose-effect relationship. The combined cytotoxic effects of 1 nmol·L-1 MCLR or MCRR and 1-100 nmol·L-1 CHClBr2 or CHCl2Br were all of additive,and the apoptosis percent was highly correlated with the exposure dose. It was suggested that the apoptosis percent of grass carp lymphocytes could be used as an effective indicator to evaluate the cytotoxicity caused by the combined pollution of MCs and DBPs.更多还原