【摘要】 目的探讨乙肝患者血清中乙型肝炎病毒大蛋白(Hepatitis B Virus Large Surface Protein,LHBs)检测的临床意义。方法采用酶联免疫吸附实验(ELISA)检测LHBs、乙型肝炎病毒标志物(HBsAg、HBsAb、HBeAg、HBeAb、HB-cAb,HBVM)、乙肝前S1Ag、乙肝前S2Ag;采用荧光定量聚合酶链反应(PCR)法检测HBV-DNA。结果29例HBsAg、HBeAg阳性患者中,LHBs与HBV-DNA阳性检出率比较,差异无显著性意义(P>0.05);乙肝前S1Ag的阳性检出率低于HBV-DNA的阳性检出率,差异有显著性意义(P<0.05);乙肝前S2Ag与HBV-DNA阳性检出率比较,差异无显著性意义(P>0.05);53例HBsAg、HBcAb阳性、HBeAg阴性患者中,LHBs与HBV-DNA阳性检出率比较,差异无显著性意义(P>0.05);乙肝前S1Ag的阳性检出率低于HBV-DNA的阳性检出率,差异有显著性意义(P<0.05);前S2Ag的阳性检出率高于HBV-DNA的阳性检出率,差异有显著性意义(P<0.05);LHBs含量与HBV-DNA拷贝数呈正相关关系(r=0.978,P<0.05)。结论LHBs可反映乙肝病毒的复制情况,特别是HBeAg阴性的患者病毒复制情况;乙肝前S1Ag和乙肝前S2Ag不适合用于判定乙肝病毒的复制水平。更多还原

【Abstract】 Objective To explore the clinical significance of HBV large protein(LHBs)in diagnosing viral replication in the serum of the patients infected with HBV.Methods LHBs,HBV preS1,HBV preS2,HBsAg、HBeAg、HBeAb and HBcAb were analyzed by ELISA.HBV-DNA was quantitatively detected using real-time PCR.Results In 29 patients whose HBsAg and HBeAg were positive,no significant difference was found in the detection rate of HBV-DNA and LHBs,PreS2(P > 0.05).Whereas the detection rate of PreS1 was lower than that of HBV-DNA(P < 0.05).In 53 patients who had positive HBsAg and negative HBeAg,.there was no significant difference between the detection rate of HBV-DNA and LHBs(P > 0.05);the detection rate of PreS1was lower than that of HBV-DNA(P < 0.05),the detection rate of PreS2 was higher than that of HBV-DNA(P < 0.05).There was a positive relationship between the level of LHBs and the amount of HBV-DNA(r = 0.978,P < 0.05).Conclusions LHBs can reflect the replication of hepatitis B virus更多还原