【摘要】 建立了芦荟中芦荟苷含量的高效毛细管电泳安培检测方法。探讨了缓冲溶液种类、浓度、酸碱度,工作电位,有机改性剂及其操作电压、进样时间等对检测的影响。Na2B4O7-NaOH(Na2B4O7浓度为30 mmol/L)为缓冲液,工作电位为-0.8 V,体积分数为2.5%甲醇为有机添加剂,进样时间为10 s,在15 kV分离高压,pH=9.5的碱性条件下柱端安培法检测了芦荟苷的含量。该法的线性范围为50~0.05 mg/L,线性方程Y=856.2+123.3c,相关系数r=0.999 4,检出限为0.01 mg/L。更多还原

【Abstract】 Barbalion in Aloe was determined by capillary electrophoresis coupled with amperometric detection(CE-AD).The pH,type and concentration of the running buffer,the detection potential,the applied voltage and the sampling time were selected as the investigated variables.Under the optimized conditions: a running buffer composed of 30 mmol/L Na2B4O7 buffer at pH 9.5,separation voltage at 15 kV,injection time 10 seconds,2.5% methanol additive and detection potential-0.8 V,a linearity existed between peak area(Y) and mass concentration of Barbalion(c) in a range of 0.05～50 mg/L,r=0.999 4,and the determination limit was 0.01 mg/L.更多还原