【摘要】 建立了人体血液中3,4-亚甲二氧基苯丙胺(MDA)、3,4-亚甲二氧基甲基苯丙胺(MDMA)的微波萃取-气相色谱(GC)测定方法。研究确定了血液中MDA、MDMA的最佳提取条件,即调节血样pH=13,以乙酸乙酯为萃取溶剂,于30℃下微波提取10 min。测定的MDA、MDMA平均萃取率分别达96.7%和101.7%,相对标准偏差分别为4.8%和5.3%(n=5),提取液经气相色谱-氢火焰离子化检测器(GC-FID)检测,2种药物和基体得到了很好的分离,对血液中MDA、MDMA的检测限为5×10-8g/mL。该方法不必对药物进行衍生化,是一种快速、准确、灵敏的测定血液中MDA、MDMA的方法。更多还原

【Abstract】 3,4-Methylenedioxyamphetamine(MDA) and 3,4-methylenedioxymethamphetamine(MDMA) in human blood were determined by means of microwave extraction-gas chromatography(GC).The optimal conditions included the pH of blood sample 13,ethyl acetate as extraction solvent,extraction at 30 ℃ for 10 min.The average recovery of MDA or MDMA with this extraction method was 96.7% or 101.7%,and the relative standard deviation of MDA or MDMA was 4.8% or 5.3%(n=5).The limit of detection was 5×10-8 g/mL for MDA and MDMA in the blood.Using this method,MDA and MDMA need not to be derivatized and can be separated from the matrix.The results indicate that the method is rapid,accurate and sensitive,and can be used for the determination of MDA and MDMA in blood samples.更多还原