【摘要】 目的探讨测定麻杏定喘合剂中苦杏仁苷含量的方法。方法采用高效液相色谱(HPLC)法,色谱柱为Dikma Kromasil C18柱(250mm×4.6mm,5μm),流动相为水-甲醇-乙腈(75:20:5),检测波长为210nm,流速为0.8mL/min,柱温30℃,进样量10μL。结果苦杏仁苷的平均加样回收率为99.02%,RSD=1.37%(n=6),平均含量为1.3575mg/g。结论所建立的方法操作简便、结果准确,可作为麻杏定喘合剂中苦杏仁苷的含量测定方法。更多还原

【Abstract】 Objective To study the method of amygdalin determination in Maxing Dingchuan Mixture.Methods HPLC was adopted with Dikma Kromasil C18 column(250mm×4.6 mm,5μm)and the mobile phase was water-methanol-acetonitrile (70:20:5).The detection wavelength was 210 nm.The flow rate was 0.8 mL/min with the temperature of 30 ℃.The sample volume was 10 μL.Results The average recovery rate of amygdalin was 99.02%with RSD of 1.37%(n=6)and the average content was 1.357 5 mg/g.Conclusion Besides simplicity,the method yields accurate and reproducible results,which can therefore be used in the quality control of amygdalin content in Maxing Dingchuan Mixture.更多还原