【摘要】 目的建立测定人血浆盐酸特比萘芬浓度的高效液相色谱法。方法以利洛司酮作内标,三氯甲烷为提取溶剂,色谱柱Acclaim C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-0.01 mol.L-1磷酸二氢钾缓冲液(pH值3.9)(5:45:50),流速1.0 mL.min-1。检测波长224 nm。结果血浆盐酸特比萘芬浓度在0.04～2.40 mg.L-1范围内线性关系良好,加权最小二乘法进行回归运算得回归方程为C=0.003 2Y+0.051 3,相关系数r=0.998 6(n=7),最低检测浓度为0.04 mg.L-1。方法回收率为99.2%～102.5%,日内RSD<12%,日间RSD<8%。结论该方法操作简便,灵敏度高,重复性好,能快速准确测定人血浆盐酸特比萘芬浓度。更多还原

【Abstract】 Objective To establish a method for determining concentration of terbinafine hydrochloride in human plasma by HPLC. Methods Lilopristone was chosen as the internal standard.Chloroform was used for extraction.Acclaim C18 column(250 mm×4.6 mm,5 μm)was used and the mobile phase was composed of methanol-acetonitrile-0.01 mol·L-1 potassium dihydrogen phosphate buffer(pH 3.9)(5:45:50),eluted at a flow rate of 1.0 mL ·min-1.UV detection wavelength was set at 224 nm Results A good linear calibration curves was obtained at the range of 0.04～2.40 mg·L-1.The regression equation was C=0.003 2 Y+0.051 3,r=0.998 6(n=7) calculated by least square method.The detection limit was 0.04 mg·L-1and the recovery was 99.2%～102.5% with intra-day RSD less than 12% and inter-day RSD less than 8%. Conclusion This method is simple,sensitive,accurate and reproducible for concentration determination of terbinafine hydrochloride in plasma.更多还原