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高效液相色谱法测定人血浆盐酸特比萘芬浓度

Determination of Terbinafine Hydrochloride in Human Plasma by HPLC

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【作者】 谭芳彭彦李瑞生张力龚培力

【Author】 TAN Fang 1,PENG Yan1,LI Rui-sheng2,ZHANG Li3,GONG Pei-li3(1.School of Pharmacy,3.Institute of Clinical Pharmacology,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;2.Department of Pharmacy,Center Hospital of Xiangfan City,Xiangfan 441021,China)

【机构】 华中科技大学同济医学院药学院湖北省襄樊市中心医院药剂科华中科技大学同济医学院临床药理研究所

【摘要】 目的建立测定人血浆盐酸特比萘芬浓度的高效液相色谱法。方法以利洛司酮作内标,三氯甲烷为提取溶剂,色谱柱Acclaim C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-0.01 mol.L-1磷酸二氢钾缓冲液(pH值3.9)(5:45:50),流速1.0 mL.min-1。检测波长224 nm。结果血浆盐酸特比萘芬浓度在0.04~2.40 mg.L-1范围内线性关系良好,加权最小二乘法进行回归运算得回归方程为C=0.003 2Y+0.051 3,相关系数r=0.998 6(n=7),最低检测浓度为0.04 mg.L-1。方法回收率为99.2%~102.5%,日内RSD<12%,日间RSD<8%。结论该方法操作简便,灵敏度高,重复性好,能快速准确测定人血浆盐酸特比萘芬浓度。

【Abstract】 Objective To establish a method for determining concentration of terbinafine hydrochloride in human plasma by HPLC. Methods Lilopristone was chosen as the internal standard.Chloroform was used for extraction.Acclaim C18 column(250 mm×4.6 mm,5 μm)was used and the mobile phase was composed of methanol-acetonitrile-0.01 mol·L-1 potassium dihydrogen phosphate buffer(pH 3.9)(5:45:50),eluted at a flow rate of 1.0 mL ·min-1.UV detection wavelength was set at 224 nm Results A good linear calibration curves was obtained at the range of 0.04~2.40 mg·L-1.The regression equation was C=0.003 2 Y+0.051 3,r=0.998 6(n=7) calculated by least square method.The detection limit was 0.04 mg·L-1and the recovery was 99.2%~102.5% with intra-day RSD less than 12% and inter-day RSD less than 8%. Conclusion This method is simple,sensitive,accurate and reproducible for concentration determination of terbinafine hydrochloride in plasma.

  • 【文献出处】 医药导报 ,Herald of Medicine , 编辑部邮箱 ,2009年12期
  • 【分类号】R96
  • 【被引频次】3
  • 【下载频次】104
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