【摘要】 目的观察熊果酸(ursolic acid,UA)诱导脑胶质瘤细胞株U251凋亡的机制。方法培养人脑胶质瘤细胞株U251,以Hoechst 33258染色法及琼脂糖凝胶电泳测定UA诱导脑胶质瘤细胞株U251凋亡的作用。免疫印记法及荧光双标法测定survivin和caspase-3表达,荧光法测定caspase-3活性。结果Hoechst 33258染色法和琼脂糖凝胶电泳显示UA明显诱导U251细胞凋亡,细胞核DNA呈梯状降解。免疫沉淀法以及荧光双标法均显示UA可抑制survivin表达;同时UA上调caspase-3表达。结论UA诱导U251细胞凋亡与其抑制survivin表达,同时上调caspase-3表达有关。更多还原

【Abstract】 Objective To explore the anti-apoptosis effect and mechanisms of Ursolic acid on human glioma U251 cell line. Methods The effect of ursolic acid on U251 cells apoptosis was observed by Hoechst 33258 staining and sepharose electrophoresis.The expressions of survivin and caspase-3 were analyzed by Western blot and double immunofluorescence staining. Results It was showed that ursolic acid remarkably induced apoptosis of U251 cell line,nucleus DNA of which was stair-like degradation.Moreover,ursolic acid suppressed survivin and upregulated caspase-3 expression. Conclusion The anti-apoptosis mechanisms of ursolic acid on human glioma U251 cell line involve in decreasing survivin expression and enhancing expression and activity of caspase-3.更多还原