【摘要】 目的研究Ouabain及其受体Na+/K+-ATPase α1、α4抗体对正常人精子运动功能的影响。方法将60例正常人的精液上游法进行优化,其中30例与不同浓度Ouabain共孵育,在1,2,3,4h采用CASA检测精子运动参数;另外30例分别与Na+/K+-ATPase α1和α4抗体共同孵育,1h后同样方法检测。结果①与阴性对照组比较,优化后的精子与较高剂量Ouabain(1×10-5～1×10-2mol.L-1)共孵育后,活动率显著下降(P<0.05),a+b级精子所占比例显著下降(P<0.01);但各浓度组两参数差异无显著性;②与阴性对照组比较,较低剂量Ouabain(1×10-6和1×10-7mol·L-1)作用后,精子活动率和a+b级精子所占比例下降均不明显,两浓度组之间差异无显著性;③Ouabain作用后,精子其他运动参数如直线速度、鞭打频率、直线性、前向性、摆动性均未见显著变化。④Anti-α1和Anti-α4作用后的精子活动率均显著下降(均P<0.01);但两者之间差异无显著性;⑤Anti-α1和Anti-α4作用后的a+b级精子所占比例均显著下降(均P<0.01);且Anti-α4作用后降低的幅度明显大于Anti-α1(P<0.05);⑥Na+/K+-ATPase α1和α4抗体作用后,精子其他运动参数如直线速度、鞭打频率、直线性、前向性、摆动性均未见显著变化。结论Ouabain在体外能够降低正常人精子运动功能;Na+/K+-ATPase α1、α4抗体也能够降低精子运动功能,但α4抗体的作用更明显。更多还原

【Abstract】 Objective To study the effects of Ouabain and it’s receptors: Na+/K+-ATPase α1 polyclonal antibody and α4 polyclonal antibody on human sperm movement. Methods 60 normal semen samples were optimized by swim-up technique.30 samples were co-incubated with high-dose(1×10-2～1×10-5 mol·L-1)and low-dose(1×10-6～1×10-7 mol·L-1) of Ouabain,respectively.The sperm movement was detected by CASA system at 1 h,2 h,3 h and 4 h.The other 30 samples were co-incubated with Na+/K+-ATPase α1 antibody and α4 antibody,respectively,the same parameters were detected in the same way 1 h later. Results ①Compared with the control group,both the ratio of sperm activity and sperm motility were decreased significantly after co-incubated with high-dose(1×10-2～1×10-5 mol·L-1)of Ouabain for 4 hour(P<0.05); ②Compared with the control group,both the sperm motility and sperm activity ratio were decreased slightly after co-incubated with low-dose(1×10-6～1×10-7 mol·L-1)of Ouabain for 4 h; And the changes among the two groups had no statistical significance yet; ③No statistical significance was found for the rest of movement parameters as linear velocity,whiplash frequency,rectilinearity,prorsad and wobble motility.④Compared with the control group,the sperm activity ratio decreased significantly after co-incubated with anti-α1 and anti-α4(P<0.01);But the changes between the two groups have no statistical significance; ⑤Compared with the control group,the sperm motility decreased significantly after co-incubated with anti-α1 and anti-α4(P<0.01);And the effect of anti-α4 on sperm motility was dramatically obvious compared with anti-α1(P<0.01); ⑥The rest of movement parameters had no statistical significance. Conclusion Ouabain can degrade the motor function of human sperm in vitro; Both anti-Na+/K+-ATPase α1 polyclonal antibody and α4 polyclonal antibody can degrade the motor function of human sperm as well,anti-α4 being better.更多还原