【摘要】 目的探讨依托咪酯对兔主动脉阻断脊髓缺血性损伤的影响。方法取日本大耳白兔24只,随机双盲均分为3组:假手术组(A组)不阻断主动脉血流;缺血再灌注组(B组)用弹性硅胶管阻断腹主动脉血流40 min;依托咪酯保护组(C组)阻断腹主动脉血流前10 min缓慢静脉注射依托咪酯乳剂0.5 mg.kg-1,续以微量输液泵持续输注0.1%依托咪酯3 mg.kg-1.h-1至松开腹主动脉时,随即阻断腹主动脉血流40 min。A、B组以同样方法静脉注射等容量0.9%氯化钠溶液作对照。监测后肢神经功能、后肢针电极肌电图(EMG)变化、脊髓组织病理学改变、脊髓匀浆丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果与A、C组比较,B组日本大耳白兔后肢神经功能评分、后肢EMG和脊髓组织形态学分级均有显著病理改变(P<0.01)。B组脊髓匀浆中MDA含量显著高于A组,而C组脊髓匀浆中MDA含量显著低于B组(P<0.01);与A组比较,B组脊髓匀浆中SOD活性明显减弱,而C组脊髓匀浆中SOD活性明显强于B组(P<0.01)。结论依托咪酯对主动脉阻断脊髓缺血-再灌注损伤具有良好的保护作用,其保护作用机制可能与具有较强的抗过氧化反应作用有关。更多还原

【Abstract】 Objective To study the effect of etomidate on spinal cord injury induced by ischemia/reperfusion(I/R). Methods Twenty four white adult Japanese rabbits were randomly assigned to 3 groups: groupA,sham-operation group(n=8);group B,ischemic reperfusion group;group C,ischemia-reperfusion with etomidate.Spinal cord ischemia was induced by infra-renal aortic cross-clamp for 40 minutes in groupB and group C.Etomidate 0.5 mg·kg-1 was intravenously injected 10 min before clamping and then infused at a rate of 3 mg·kg-1·h-1 until unclamping in group C,0.9% saline was used as control in other groups.After surgical operation,post-operative neurological function,electromyography of rear limbs,histopathology,malondialdehyde(MDA) levels and superoxide dismutase(SOD) activities in the spinal cord were assessed for all animals. Results Compared with group A,group B showed significant I/R injury-induced changes in the neurological function scores,histopathology and electromyography(P<0.01).However,groups C treated with etomidate significantly reversed these changes of all parameters(P<0.01).Meanwhile,I/R-induced increase in MDA content observed in group B were also significantly reduced in group C(P<0.01),and I/R-induced decrease in the SOD activities were also significantly prevented in etomidate-treated group(P<0.01). Conclusion Etomidate showed potent protective effects against spinal cord I/R injury in the rabbit model,and protected loss of antioxidant activities of the spinal cord as well.更多还原