【摘要】 目的:使用高纯化的人外周血单核细胞研究蛋白酶激活受体(protease activated receptors,PARs)在单核细胞上的表达。方法:采用密度梯度离心法和磁激活细胞分选法分离人外周血单核细胞,采用流式细胞仪、逆转录聚合酶链反应(RT-PCR)分析单核细胞PARs表达情况。结果:流式细胞分析示人外周血单核细胞表达PAR-1、PAR-3、PAR-4蛋白质,但不表达PAR-2蛋白质;RT-PCR示单核细胞表达PAR-1和PAR-3,但不表达PAR-2和PAR-4 mRNA。结论:单核细胞表达PARs,为进一步研究PARs在单核细胞上的功能提供依据。更多还原

【Abstract】 Objective:To explore the expression of protease activated receptors(PARs) on highly purified human monocytes.Methods:Flow cytometry and RT-PCR were used to detect the expressions of PARs on human monocytes that purified by magnet-activated cell sorting.Results:Flow cytometry analysis showed that monocytes express PAR-1,PAR-3,PAR-4 protein,but not PAR-2.RT-PCR revealed that monocytes express PAR-1 and PAR-3,but not PAR-2 and PAR-4 mRNA.Conclusion:PARs are expressed on human monocyte cells,which may facilitate further investigation of the potential functions of PARs on monocytes.更多还原