【摘要】 目的:研究人参瓜蒌莪术汤(RGE)对Lewis肺癌模型小鼠的治疗作用及机制。方法:建立小鼠Lewis肺癌移植瘤模型,分为模型对照组(NS组),环磷酰胺对照组(CP组),RGE高、中、低剂量组。在计算抑瘤率的基础上,运用PCR-ELISA法检测瘤组织端粒酶活性。结果:RGE低、中、高剂量组均能明显降低小鼠Lewis肺癌瘤重(P<0.01),中剂量组抑瘤率达47.88%;RGE低、中、高剂量组均能明显降低瘤组织细胞端粒酶的活性(P<0.01),中剂量组对端粒酶活性的抑制作用显著强于CP组(P<0.01)。结论:RGE具有抑制小鼠Lewis肺癌生长的作用,其作用机制可能与降低肿瘤组织端粒酶活性有关。更多还原

【Abstract】 Objective:To explore the influence of treatment and mechanism of Renshengualouezhu Decoction(RGE) on Lewis lung cancer in mice.Methods:Mice Lewis lung cancer models were established,and divided into 5 groups:model control group(NS group),low-dose RGE group,middle-dose RGE group,high-dose RGE group and Cyclophosphamide group(CP group).Then calculated the inhibition rate and used PCR-ELISA to detect the activity of telomerase.Results:The tumor weight of low-dose RGE group,middle-dose RGE group,high-dose RGE group and CP group were significantly decreased(P<0.01).Inhibition rate in the middle-dose RGE group reached 47.88%.RGE could reduce the telomerase activity on all dose levels of low,middle or high(P<0.01).The middle-dose RGE group was significantly better than the CP group(P<0.01).Conclusion:RGE can significantly inhibit the growth of mice Lewis lung cancer.It may be related to its ability to reduce telomerase activity.更多还原