【摘要】 目的:探讨人参皂苷化学修饰物PM1体外诱导肝癌HepG2细胞凋亡作用。方法:采用四甲基偶氮唑蓝(MTT)还原法检测不同浓度的人参皂苷化学修饰物PM1对肝癌HepG2细胞增殖的作用;流式细胞仪分析肿瘤细胞凋亡周期及细胞凋亡率。结果:PM1抑制肝癌HepG2细胞生长,呈浓度依赖关系。用50和100mg/LPM1处理细胞24h后,PM1诱导了细胞凋亡和调节细胞周期,凋亡率与对照组比较明显升高(P<0.01)。结论:PM1抑制肝癌HepG2细胞生长,其机制可能是通过诱导凋亡来发挥作用的。更多还原

【Abstract】 Objective:To study the effect of PM1 on inducing apoptosis of HepG2 cells in vitro.Methods:The HepG2 cells were treated with different doses of PM1.Cell proliferation was measured by MTT assay.Cell cycle and apoptosis rate were analyzed by flow cytometry.Results:PM1 inhibited proliferation of HepG2 cells in concentration-dependent manner.FCM assay showed that PM1 regulated cell cycle and induced apoptosis of HepG2 cells after treated with 50 and 100 mg/L PM1 for 24 h.The apoptosis rates were increased(P<0.01).Conclusion:PM1 probably inhibits the proliferation of HepG2cells by inducing apoptosis.更多还原