【摘要】 目的:研究白藜芦醇对大鼠局灶性脑缺血再灌注损伤抗炎作用机制。方法:大鼠脑缺血再灌注模型完成后,缺血2h再灌注24h,其中白藜芦醇大剂量组(40mg/kg)、小剂量组(10mg/kg)术前7d分别腹腔注射2ml/kg白藜芦醇无菌溶液,模型组腹腔注射2ml/kg生理盐水,1次/d,各组大鼠均于手术前15min加注1次。观察不同剂量的白藜芦醇对脑组织一氧化氮(NO)含量、髓过氧化物酶(MPO)活性和脑组织神经细胞的细胞间黏附分子-1(ICAM-1)蛋白的表达的影响。结果:白藜芦醇可显著减轻大鼠脑缺血再灌注损伤的行为评分,显著降低脑组织中NO含量和MPO的活性,差异有统计学意义(P<0.05);ICAM-1蛋白的阳性表达率降低,差异有统计学意义(P<0.05)。结论:白藜芦醇对大鼠脑缺血再灌注损伤有保护作用,作用机制之一可能与其抑制炎性反应有关。更多还原

【Abstract】 Objective:To investigate the anti-inflammatory mechanisms of resveratrol on cerebral ischemia reperfusion in-jury(CIRI) of rats.Methods:The model of MCAO was established by intraluminal suture technique.After 2 h of occlusion and 24 h of reperfusion,the rats of the high dose resveratral(40 mg/kg) group and low dose resveratrol(10 mg/kg) group were injected by intraperitoneal injection for 7 days,respectively,and the control group were given normal saline(2 ml/kg),one time a day,and all groups were given a more time in 15 minutes before operation.The contents of NO and activities of MPO in brain tissue of ischemic hemisphere were measured;the expression of ICAM-1 in the cortex were measured by immunohistochemical method.Results:The neurological scores of rats treated with resveratrol was reduced apparently compared with model group rats(P<0.05);there were apparent reduction of the contents of NO and marked decrease of MPO in Resveratrol group compared with the I/R model group(P<0.05);compared with I/R model group,the expression of ICAM-1 was down-regulated obviously in resveratral group(P<0.05).Conclusion:Resveratrol could relieve the cerebral ischemia reperfu-sion injury,one of the mechanisms could be the anti-inflammatory effect of procyanidin更多还原