【摘要】 目的:观察抗癌中药"卡宁"对体外培养的肝癌细胞株BEL-7402细胞的凋亡抑制基因bcl-2的影响,探讨其抗肿瘤治疗的作用机制。方法:取体外培养的对数生长期BEL-7402细胞,随机分为4组:对照组,5-氟尿嘧啶组,含药"卡宁"血清1组(10%含药血清组)和含药"卡宁"血清2组(15%含药血清组);采用MTT比色法观察不同浓度含药血清对体外培养的人肝癌细胞株BEL-7402细胞的抑制能力,PCR检测其对凋亡抑制基因bcl-2的影响。结果:PCR结果显示,"卡宁"1、2组bcl-2的活性明显低于对照组(P=0.038);MTT结果显示,细胞生长抑制率随含药血清作用时间及浓度的增加而增大(P<0.05),15%含药血清72h的作用最强。结论:对肿瘤细胞bcl-2的抑制可能是抗癌中药"卡宁"诱导肿瘤细胞凋亡作用机制的一部分。更多还原

【Abstract】 Objective:To observe the effects of anticancer chinese medicine "Caning" on the anti-apoptosis gene bcl-2 of in vitro cultured human hepatic cancer cell line BEL-7402 cells,and investigates its anticancer therapeutic mechanism.Methods:In vitro cultured BEL-7402 cells in a logarithmic growth phase were randomly divided into 4 groups:control group,5-Fluorouracil treated group,serum group 1 containing "Caning"(10% pastille serum) and serum group 2 contain-ing "Caning"(15% pastille serum);then MTT assay was employed to detect the inhibitory ability of serums containing dif-ferent concentrations of "Caning" to in vitro cultured human hepatic cancer cell line BEL-7402 cells,and PCR was used to detect the effect of "Caning"on the anti-apoptosis gene bcl-2.Results:Results of in vitro experiment suggested that,the bcl-2 gene activity in serum group 1 and 2 containing "Caning" was significantly lower than that of control group(P=0.038);result of MTT assay indicated that the cell growth inhibitory rate was increased along with the increase of the action time and concentration of serum containing "Caning"(P<0.05),and the 15% pastille serum showed the strongest inhibitory effect at 72 h.Conclusion:"Caning"could significantly inhibit the growth of hepatic cancer cells partly by inhibiting the anti-apoptosis gene bcl-2.更多还原