【摘要】 目的观察2型糖尿病老年患者外周血中内皮细胞(CECs)、内皮祖细胞(EPCs)和循环祖细胞(CPCs)改变,探讨内皮功能紊乱在糖尿病视网膜微血管病变发生发展中的作用。方法选择糖尿病老年患者30例和老年正常对照10例,流式细胞仪测定外周血中CECs、EPCs和CPCs含量。根据有无视网膜微血管病变分为糖尿病组(DM)、背景型糖尿病视网膜微血管病变组(NPDR)和增生型糖尿病视网膜微血管病变组(PDR),并进行糖尿病视网膜微血管病变与内皮功能紊乱相关性分析。结果(1)DM患者外周血中CECs、EPCs和CPCs的含量分别为0.145%±0.006%、0.059%±0.004%、0.12%±0.03%。DM+DR患者外周血中CECs、EPCs和CPCs的含量分别为0.185%±0.013%、0.043%±0.004%、0.05%±0.02%。15例正常人外周血中CECs、EPCs和CPCs的含量分别为0.067%±0.013%、0.062%±0.004%、0.156%±0.02%,DM患者外周血中的ECs含量均高于正常对照组(P<0.05),EPCs和CPCs的含量低于正常对照组(P<0.05)。(2)PDR与NPDR组rBDR与DM组=0.221,P<0.05;rPDR与NPDR组=0.354,P<0.05;EPCs与微血管病变程度相关系数rBDR与DM组=-0.121,P<0.05;rPDR与NPDR组=-0.175,P<0.05。CPCs与微血管病变程度相关系数rBDR与DM组=-0.373,P<0.05;rPDR与NPDR组=-0.361,P>0.05。(3)相关性分析显示在2型糖尿病组中,EPCs数值与BMI、腰臀比、腹围、FPG,TG,UAER,肌酐,尿酸有负相关性(r值分别为-0.218,-0.223,-0.208,-0.145,-0.126,-0.271,-0.224,-0.198,P<0.05)。结论糖尿病患者外周血CECs、EPCs、CPCs)与微血管病变有一定的相关性,三者作为糖尿病患者血管病变发生发展的早期变化预测指标可能有一定意义。更多还原

【Abstract】 Objective The changes of CECs,EPCs and CPCs in the old type 2 diabetes mellitus retinopathy(DR)patients and the clinical purpose were discussed.Methods 30 cases of T2DM(including 10 DM cases without complications and 20 DM cases with retinopathy)and 10 cases of health controls were enrolled in this study.Results(1)The changes of CECs in DR Patients were significantly higher while the levels of EPCs and CPCs were significantly lower than those in the patients of the control group.(2)There were some corrlation beteen the level of CECs,EPCs and CPCs and the degree of diabetic retinopathy.(3)The serum EPCs levels in type 2 diabetics were negativey associated with body mass index,waist-hip ratio,abdominal circumference,FPG,triglyceride,UAER,creatinine.Conclusion The levels of EPCs and CPCs in T2DM-DR patients may be the important detected targets for early discovery and monitoring of DR.更多还原