【摘要】 目的通过体外葡萄球菌生物被膜(BF)的培养、诱导和观察,研究磷霉素(FOS)对BF的作用。方法选取临床分离葡萄球菌和质控菌株ATCC25922,ATCC25923采用胰酶肉汤培养基(TSB-teflon)系统和生理盐水(NS-teflon)系统进行BF的培养、诱导和聚焦显微镜观察BF、琼脂二倍稀释法分别测定药物的最低抑菌浓度(MIC)。结果经过1、3、7天的孵育,临床分离菌的生物被膜的堆积随着时间的延长而增多;加入磷霉素后生物被膜的生长受到抑制。有无生物被膜组、有无磷霉素组MIC差别显著(P<0.05)。结论磷霉素在体外对葡萄球菌BF有抑制作用。更多还原

【Abstract】 Objective Through the training,induction and observation,of biofilm Staphylococcus aureus(BF)in vitro to study fosfomycin(FOS)on the role of BF.Methods Select the clinical and quality control strains of Staphylococcus ATCC25922,ATCC25923 using trypsin broth medium(TSB-teflon)systems and saline(NS-teflon)system for the training,induction and confocal microscope observation of BF.The minimum inhibitory concentration(MIC)was determined by twice the agar dilution Drugs.Results After 1,3,7 d of incubation,clinical isolates of the biofilm accumulation over time of the extension increased After joining fosfomycin biofilm,the growth was inhibited.There was significant difference in MIC with or without biofilm(P<0.05).Conclusion Fosfomycin in vitro inhibited Staphylococcus BF.更多还原