【摘要】 目的探讨粒细胞-巨噬细胞集落刺激因子(GM-CSF)、C反应蛋白(CRP)和肿瘤坏死因子α(TNFα)在婴儿巨细胞病毒(CMV)肝炎发病中的作用。方法56例CMV肝炎患者和51例正常对照者,用荧光探针PCR法定量检测血液中巨细胞病毒DNA(HCMV-DNA),用双抗体夹心法ELISA检测血清中的GM-CSF和TNFα水平,用免疫比浊法检测血液中CRP含量。结果婴儿巨细胞病毒肝炎患者血清中GM-CSF、CRP和TNFα水平明显高于正常对照组(P<0.01,P<0.01,P<0.01)。结论GM-CSF、TNFα和CRP参与婴儿巨细胞病毒肝炎的免疫病理反应和抗病毒免疫。更多还原

【Abstract】 Objective To explore the role of granulocyte macrophage colonystimulating factor(GM-CSF),C reaction protein(CRP)and tumor necrosis factor-αlpha(TNFα)in infant cytomegalovirus hepatitis.Methods Cytomegalovirus DNA of 56 cases of children with cytomegalovirus hepatitis and 51 normal controls in blood were detected using FQ-PCR.The levels of GM-CSF and TNFαin serum were assayed with ELISA,and CRP was measured with immunoturbidmemetry assay.Results The levels of M-CSF,CRP and TNFα in serum of infant cytomegalovirus hepatitis were higher obviously than those in serum of the normal control group.Conclution M-CSF,CRP and TNFα participate in pathological immune response and anti-infective immunity of cytomegalovirus hepatitis.更多还原