【摘要】 目的探讨不同样品制备方法对结肠癌细胞表面sialyl LewisA/X抗原的影响。方法应用EDTA、胰酶、细胞刮、胰酶-EDTA、胰酶联用细胞刮刀组制备培养人结肠癌细胞单细胞悬液。采用流式细胞仪检测人结肠癌细胞表面sialyl LewisA/X的表达和细胞数。结果单用EDTA或细胞刮制备的细胞悬液中细胞产量低,胰酶或胰酶联用细胞刮刀组制备的细胞悬液中细胞产量高。胰酶组细胞表面sialyl LewisA/X的荧光强度明显降低。EDTA组、细胞刮组、胰酶联合细胞刮组间sialyl LewisA/X的荧光强度表达差异无统计学意义。结论胰酶联用细胞刮法是贴壁培养细胞制备单细胞悬液的一种好方法。更多还原

【Abstract】 Objective To investigate the effects of variable sample preparation about sialy LewisA/X on the surface of colon carcinoma cells.Methods Human colon carcinoma cell suspension was prepared by using EDTA,trypsin,cell scraper,trypsin-EDTA and trypsin-cell scraper.Cell counts and sialy-LewisA/X on the surface of colon carcinoma cells were detected by flow cytometry.Results Trypsin made fluorescence intensity of sialy-LewisA/X on the surface of colon carcinoma cells become weaken.Fluorescence intensity of sialy-LewisA/X had no significant deviation among EDTA,cell scraper and trypsin-cell scraper.Cell counts were multitude in EDTA group or trypsin-cell scraper group.Conclusion Trypsin-cell scraper is a available approach on preparing cell suspension of adherent cell.更多还原