【摘要】 目的分析不同固定剂对冰冻切片免疫组化的影响,寻找出最佳的冰冻切片免疫组化的固定剂。方法取常见的组织或肿瘤6种,冰冻切片后,置于6种不同冰冻固定液,95%酒精、4%甲醛、甲醇、丙酮、EAF(配方为无水乙醇85mL、冰醋酸5mL、40%甲醛10mL)、AAF(配方为丙酮85mL、冰醋酸5mL、40%甲醛10mL),固定5min,根据组织含有的特定抗原进行相应抗体的免疫组化染色,结果采用中国病理学工作者委员会免疫组化质控中心推荐的免疫组化质控评分标准进行评分。结果不同的固定剂可直接影响到冰冻切片免疫组化染色的成败和质量的高低,甚至会造成假阴性;混合固定液EAF优于其它固定液,其它五种固定液的染色效果依次为AAF、95%酒精、4%甲醛、甲醇、丙酮。结论用冰冻切片做免疫组化染色,对于大多数抗体而言,EAF固定液的效果优于其他5种固定液。更多还原

【Abstract】 Objective To evaluate the influence of different fixatives on immunohistochemical staining of frozen sections and to find the most suitable fixative for immunohistochemical staining of frozen sections.Methods Immunohistochemical staining was done for frosen sections which were respectively fixed for 5 min in 6 different fixatives,including 95% alcohole,4% formaldehyde,methanol,acetone,EAF(containing 85% absolute ethanol,5% glacial acetic acid and 4% formaldehyde) and AAF(containing 85% acetone,5% glacial acetic acid and 4% formaldehyde).The staining results were analyzed with the scoring standards recommended by the Immunohistochemistry Quality Control Center,Committee Chinese Pathologists.Results Different fixative could make direct influence on the quality of immunohistochemical staining for frozen sections and even caused false negative results.Compared with the other 5 fixatives,mixed fixative EAF contributed to better immunohistochemical staining.Conclusion EAF solution was more suitable to be used as a fixtive for immunohistochemical staining of frozen sections than the other 5 fixtives.更多还原