【摘要】 核磁共振代谢组学法研究大蒜辣素对大鼠的作用机制。以生物核磁共振技术结合偏最小二乘-辨别分析法(PLS-DA)分析大鼠腹腔注射大蒜辣素后尿液内源性代谢产物的变化,研究大蒜辣素对正常大鼠代谢过程的影响。给药后,尿样中三羧酸循环中间产物柠檬酸、α-酮戊二酸、琥珀酸含量呈现出先升高后逐渐恢复的趋势,酮体水平也有所上升;而葡萄糖、乳酸、丙氨酸、马尿酸、氮氧三甲胺和牛磺酸水平略有降低。偏最小二乘分析表明,给药组与对照组的代谢谱有明显差异。大蒜辣素给药后对正常大鼠代谢过程具有显著影响,代谢组学研究有助于认识大蒜的作用机制。更多还原

【Abstract】 To investigate the effects of allicin on rats by NMR-based metabonomic method,the changes of endogenetic metabolites in normal rat urine and the influences on metabolism were analyzed with bio-nuclear magnetic resonance(NMR) method and partial least-squares discriminant analysis(PLS-DA) after intraperitoneal administration of allicin solution.The identified biochemical effects associated with allicin dosing included elevated then gradually recovered urinary levels of Kreb’s cycle intermediates,such as citrate,α-ketoglutarate and succinate and increased concentrations of ketones.Meanwhile,decreased urinary concentrations of glucose,lactate,alanine,hippurate and trimethylamine oxide were observed.The PLS-DA revealed that the matabonomic profiles of allicin treated groups were obviously different from those of the control group.Allicin may change metabolism significantly in normal rats.The study of the pharmacologic mechanism of allicin by metabonomic method is practicable and it could be explored further.更多还原