【摘要】 [目的]探讨山东地区HBV基因型的分布情况,研究不同HBV基因型感染者的临床特点及病毒学特点。[方法]设计针对HBV S基因片段扩增的引物,采用PCR-RFLP的基因分型方法,对128例HBV感染者进行了HBV基因分型并检测了HBV前C及基本核心启动子(BCP)变异。[结果]128份血清中,基因C型104例,占81.25%;基因B型22例,占17.19%,未检测到其它基因型。有2份血清未能明确分型。[结论]山东地区HBV基因型以C型为主,其次为B型。HBV基因C型与基因B型比较有显著不同的临床特点。在慢性HBV携带者中基因C型的比例明显低于慢性肝炎、重型肝炎及肝细胞癌患者;HBV基因B型患者的平均年龄明显小于基因C型。HBV基因C型与基因B型比较有显著不同的病毒学特点。HBV DNA基因C型感染者的HBeAg阳性率、病毒载量及BCP变异率均明显高于基因B型。提示有可能基因B型更容易出现HBeAg的血清转换,高HBV复制水平的基因C型更容易导致反复的肝脏炎症活动。不同HBV基因型的病毒学特点部分决定了其临床特点。更多还原

【Abstract】 [Objective]To explore the distribution of Hepatitis B virus(HBV) Genotype in Shandong Province and the clinical,virological features of the patients infected with different HBV genotype.[Methods]The HBV Genotype,Pre C and Basal core promoter(BCP) mutations of 128 chronic HBV infected patients were detected using PCR-RFLP methods.[Results]The prevalence rate of HBV genotype C(104) and Genotype B(22)in Shandong Province was 81.25% and 17.19% respectively.No other Genotype was detected.[Conclusion]HBV Genotype C is the predominant Genotype in Shandong Province.There exists clinical and virological difference between patients infected with HBV genotype B and C.The prevalence rate of HBV genotype C in chronic HBV carrier is lower than those in chronic Hepatitis B,cirrhosis and hepatocellular carcinoma patients.The age of patients infected with HBV Genotype B is younger than those infected with Genotype C.The HBeAg positive rate,HBV DNA level and BCP mutation rate in Genotype C is higher than that in Genotype B.It was suggested that HBeAg seroconversion can be easily detected in Genotype B,Genotype C which had high level of HBV duplication can result to repeated inflammation in the liver.The clinical feature was based on virological characteristic of different HBV genotypes.更多还原