【摘要】 应用戊二醛法偶联HM-3与BSA,合成完全抗原,免疫新白兰白兔,制备HM-3多克隆抗体。同位素示踪法测定多抗效价、特异性和亲和力。结果表明:抗体的效价为1∶2000;亲和力常数经Scatchard分析计算为2.84×109L/mol,达到高敏分析要求;交叉实验结果表明抗体有较高的识别特异性。HM-3多抗的制备为放射免疫分析方法的建立奠定了基础。更多还原

【Abstract】 In order to prepare the polyclonal antibody of HM-3,glutaral coupling was used to couple HM-3 with the carried protein BSA to obtain synthetic antigen.The anti-HM-3 serum was produced by immunizing New Zealand rabbits.Polyclonal antibody’s valence,specificity and affinity were detected by Isotope tracer method.The results showed that the molecular conjugate ratio between BSA and HM-3 is 1∶30.The antibody possessed titer of 1∶2000 and affinity constant(ka) of 2.84×109L/mol according to Scatchard analysis,which was quite adequate;cross reaction test showed high specificity.This has laid the basis for RIA of HM-3.更多还原