【摘要】 目的:建立薄层色谱原位预处理-薄层色谱法鉴别药材中的齐墩果酸和熊果酸。方法:选取中国药典2005年版一部收载的、以齐墩果酸或熊果酸为鉴别指标的11味中药材,取供试液适量分别点于同一薄层板上,将点样斑点首先以1%碘-二氯甲烷溶液进行预处理,再以环己烷-二氯甲烷-乙酸乙酯-冰醋酸(20∶5∶8∶0.1)为展开剂展开,以10%硫酸乙醇溶液为显色剂,105℃加热显色,日光检视。结果:此方法可同时检出药材中含有齐墩果酸和熊果酸的情况,齐墩果酸和熊果酸Rf值分别为0.54和0.38。结论:本方法适用于分离鉴别药材中的齐墩果酸与熊果酸,为质量标准的制定提供了新的思路。更多还原

【Abstract】 Objectives:Create an in-situ pretreatment TLC method to separate oleanolic acid and ursolic acid in Chinese material medicas collected in ChP 2005,which use oleanolic acid or ursolic acid as an indicator to test.Method:The samples were treated directly on the chromatographic plate by 1% iodine solution in methylene dichloride and developed with the mobile phase consisting of cyclohexane-methylene dichloride-ethyl acetate-glacial acetic acid (20∶5∶8∶0.1).The plates were sprayed with 10% ethanol solution of sulfuric acid and heated to 105 ℃ for 5 min.Results:Oleanolic acid and ursolic acid were detected in the Chinese material medicas that contain them.The Rf of Oleanolic acid and ursolic acid were 0.54 and 0.38,and they were effectively separated.Conclusion:The results were found to be satisfactory.The method can be used to identify oleanolic acid and ursolic acid in Chinese material medicas simultaneously.更多还原