【摘要】 目的:建立RP-HPLC法测定不同产地、不同种属郁李仁中苦杏仁苷含量。方法:采用Kromasil C18柱(5μm,250mm×4.6mm);以乙腈-水(12∶88)为流动相,流速1.0mL·min-1;检测波长210nm;柱温35℃。结果:苦杏仁苷的线性范围为5.040～80.6μg·mL-1(r=0.9999);平均回收率(n=9)为99.8%。结论:本法简便,结果准确,重复性好,可作为测定不同种属郁李仁药材中苦杏仁苷含量的方法。更多还原

【Abstract】 Objective:To establish an RP-HPLC method for the determination of amygdalin in Semen Pruni of different species and different areas.Methods:The chromatographic method was carried out on Kromasil C18column(5 μm,250 mm×4.6 mm) using acetonitrile-water(12∶88) as the mobile phase;The flow rate was 1.0 mL·min-1;the detection wavelength was set at 210 nm,and the column temperature was 35 ℃.Results:The linear range of amygdalin was 5.040-80.6 μg·mL-1(r=0.9999),and the average recovery(n=9) was 99.8%.Conclusion:The method is simple,accurate with good reproducibility.It can be applied in quantitative of determination of amygdalin in Semen Pruni.更多还原