【摘要】 目的:建立RP-HPLC法同时测定夏枯草不同部位中熊果酸和齐墩果酸含量。方法:采用SHIMADZUVP-ODS柱(4.6mm×250mm,5μm),以乙腈-甲醇-水-醋酸铵(69∶16∶15∶1)为流动相,流速1.0mL·min-1,检测波长210nm。结果:熊果酸、齐墩果酸进样量分别在0.780～6.240μg及0.756～6.048μg范围内线性关系良好,熊果酸、齐墩果酸平均回收率(n=6)分别为99.2%(RSD=0.69%)和99.1%(RSD=1.2%)。结论:本法简便、准确、灵敏,重复性好,可用于夏枯草不同部位熊果酸和齐墩果酸的含量测定。更多还原

【Abstract】 Objective:To establish an RP-HPLC method for the determination of ursolic acid and oleanolic acid in different parts of Prunella vulgaris L.. Methods:The determination was carried out with SHIMADZU VP-ODS column(4.6 mm×250 mm,5 μm),using acetonitrile -methanol-water-ammonium acetate(69∶ 16∶ 15∶ 1) solution as mobile phase at a flow rate of 1 mL·min-1 and the detection wavelength was at 210 nm. Results:The linear ranges of ursolic acid and oleanolic acid were 0.780-6.240 μg and 0.756-6.048 μg,respectively. The average recoveries(n=6) of ursolic acid and oleanolic acid were 99.2 % with RSD 0.69 % and 99.1% with RSD 1.2 %,respectively. Conclusion:The method is simple,accurate,reproducible and can be used for determining ursolic acid and oleanolic acid in different parts of Prunella vulgaris L..更多还原