【摘要】 目的:建立木犀草素血药浓度的高效液相色谱测定方法,并探讨其在大鼠体内的药动学行为。方法:采用Agilent TC-C18(4.6mm×150mm,5μm)色谱柱,以乙腈-0.5mol·L-1磷酸二氢钾溶液(40∶60)为流动相,流速1.0mL.min-1,检测波长280nm。大鼠灌胃木犀草素30mg·kg-1后,于不同时间点断尾采血,用HPLC法测定其血药浓度,经3P97药动学计算程序处理,得到药动学参数。结果:大鼠血浆中,木犀草素的线性范围为20～1200ng·mL-1(r=0.9967),方法回收率大于96%,最低检测限为15ng·mL-1。大鼠灌胃木犀草素后,木犀草素的血药浓度-时间变化曲线符合二室模型,Tmax为(0.732±0.13)h,Cmax为(0.691±0.14)mg·mL-1,AUC为(0.435±0.12)mg·h·mL-1。结论:本试验建立的大鼠血浆中木犀草素含量的HPLC测定方法,专属性强,灵敏度高,可用于该药在大鼠体内的药代动力学研究。更多还原

【Abstract】 Objective:To establish an HPLC method for the determination of luteolin in rat plasma and to study the pharmacokinetics of luteolin in rats.Methods:An Agilent TC-C 18(4.6 mm×150 mm,5 μm) column was adopted,the mobile phase was a mixture of acetonitrile and 0.5 mol·L-1 potassium dihydrogen phosphate solution(40∶60) at the flow rate of 1.0 mL·min-1 and the UV detector was operated at 280 nm.After oral administration of luteolin(30 mg·kg-1),the luteolin concentration in rat plasma was detected by HPLC.The pharmacokinetic parameters were calculated by 3P97 program.Results:In rat plasma,a good linearity between peak area ratio of diosmetin to the internal standard and the concentration was found in the range of 20-1200 ng·mL-1(r=0.9967);the method recoveries were over 96%.The limit of test was 15 ng·mL-1.The plasma luteolin concentration-time curve could be evaluated by the two compartment model. T max,C max and AUC were (0.732±0.13) h,(0.691±0.14) mg·mL-1 and (0.435±0.12) mg·h·mL-1,respectively.Conclusion:The developed HPLC method for the pharmacokinetic study of luteolin in rat after oral administration is precise and specific.更多还原