【摘要】 目的:采用高效液相色谱法测定匹诺塞林的含量及其有关物质。方法:采用Kromasil C18色谱柱(4.6mm×150mm,5μm);以甲醇-水(用磷酸调pH至3.0)(63∶37)为流动相;流速1.0mL·min-1;检测波长:290nm;柱温:室温;进样量:20μL。结果:高效液相色谱法测定的线性范围为2~200μg.mL-1;相关系数r=1.0000;日内精密度:RSD为0.10%(n=5);日间精密度:RSD为0.14%(n=5)。杂质1、2、4的线性范围为0.05~50μg.mL-1;相关系数r分别为1.0000,1000,0.9999。杂质3的线性范围为0.2~8μg.mL-1;相关系数r=0.9999。结论:本法简便快速、准确、专属性好。更多还原

【Abstract】 Objecfive:To established an HPLC method for the determination of pinocembrin and its related substances.Method:The separation was performed on a Kromasil C18 column(4.6 mm×150 mm,5 μm).The mobile phase was composed of methanol-water(adjusted to pH 3.0 with phosphoric acid)(63∶37)at the flow rate of 1.0 mL·min-1.The detection was carried out with a UV detector at 290 nm.The chromatography was conducted at room temperature.The injection was 20 μL.Results:The calibration curves were linear between 2-200 μg·mL-1(r=1.0000).Intra-day and inter-day RSDs were 0.10%(n=5)and 0.14%(n=5).The calibration curves of imputrity 1,impurity 2,impurity 4 were linear between 0.05-10 μg·mL-1,r=1.0000,1.0000,0.9999,respeutinely.The calibration curves of impurity 3 was linear between 0.2-8 μg·mL-1(r=0.9999).Conclusion:The method is simple,rapid,accurate and is suitable for the determination of pinocembrin and its related substances.更多还原