【摘要】 目的:RP-HPLC法同时测定黄芪药材中6个黄酮类成分的含量。方法:采用Diamonsil C18柱(250mm×4.6mm,5μm),流动相为乙腈-0.05%磷酸水溶液梯度洗脱,检测波长为210nm和250nm,柱温35℃。结果:芒柄花素在3.31～33.12μg·mL-1(r=0.9996)、芒柄花素-7-O-β-D-葡萄糖苷在2.52～25.20μg·mL-1(r=0.9992)、毛蕊异黄酮在6.64～66.40μg·mL-1(r=0.9992)、毛蕊异黄酮-7-O-β-D-葡萄糖苷在10.80～108.0μg·mL-1(r=0.9994)、(6αR,11αR)-9,10-二甲氧基紫檀烷-3-O-β-D葡萄糖苷在2.42～24.16μg·mL-1(r=0.9993)和2′-羟基-3′-,4′-二甲氧基异黄烷-7-O-β-D-葡萄糖苷在2.79～27.90μg·mL-1(r=0.9994)范围内峰面积与浓度呈良好的的线性关系(n=6)。6种成分的加样回收率均高于95.0%,RSD小于2.9%。结论:方法简便、快速、准确,重现性好,为黄芪药材的质量控制提供依据。更多还原

【Abstract】 Objective:To develop an RP-HPLC method for the simultaneous determination of six flavonoids in Radix Astragali.Methods:The reversed phase HPLC system consisting of a Diamonsil C18 column (250 mm×4.6 mm,5 μm ) and a mixture of acetonitrile and 0.05% phosphoric acid as the mobile phase was adopted with gradient elution. The column temperature was set at 35 ℃ and the flow rate was 1.0 mL·min-1. The absorbance was monitored at 210 nm and 250 nm.Results:The linear response ranges were 3.31-33.12 μg·mL-1(r = 0.9996) for formononetin,2.52-25.20 μg·mL-1(r=0.9992) for formononetin-7-O-β-D-glucoside,6.64 -66.40 μg·mL-1(r=0.9992) for calycosin,10.80-108.0 μg·mL-1(r=0.9994) for calycosin-7-O-β-D-glucoside,2.42-24.16 μg·mL-1(r=0.9993) for 9,10-dimethoxypterocarpan-3-O-β-D-glucoside and 2.79-27.90 μg·mL-1(r=0.9994) for 2’-hydroxy-3’,4’-dimethoxy-isoflavane -7-O-β-D-glucoside ,respectively (n=6). The average recoveries (n=9) of six flavonoids were greater than 95.0%,and RSD were less than 2.9%.Conclusion:The results demonstrated that the method had adequate accuracy and selectivity to measure the concentrations of six flavonoids in Radix Astragali.更多还原